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Heat inactivation: No.
Ligation and recutting: After ten-fold overdigestion with Bcl I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Large excess of the enzyme results in the appearance of star activity.
DNA Methylation:
No inhibition: dcm, CpG
Inhibition (Blocked): dam
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (dam-; 8 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
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