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Heat inactivation: No.
Ligation and recutting: After 30-fold overdigestion with Bgl II, >95 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation: No inhibition: dam, dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (6 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
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