BseB I

Recognition Sequence:

Heat inactivation: No.

Ligation and recutting: After ten-fold overdigestion with BseB I,
<50 % of the DNA fragments can be ligated.

Star activity: Low salt concentration or large excess of enzyme results in the appearance of star activity.

Note: BseB I-cut DNA is difficult to ligate with T4 DNA Ligase. Ligation is enhanced in the presence of 15 % PEG 4000.

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (70 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.