BseC I

Recognition Sequence:

Heat inactivation: No.

Ligation and recutting: After 30-fold overdigestion with BseC I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.

DNA Methylation:
No inhibition: dcm
Inhibition (Blocked): CpG
Inhibition (Blocked by overlapping): dam

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (15 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.