BstE II

Recognition Sequence:

Heat inactivation: No.

Ligation and recutting: After forty-fold overdigestion with BstE II, >95 % of the DNA fragments can be ligated and recut with this enzyme.

Note: BstE II exhibits 10 - 15 % activity at 37°C.

DNA Methylation: No Inhibition: dam, dcm, CpG

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (13 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.