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EcoR V

Datasheet
НазваниеКодЦена Количество
L pack EN-115L по запросу 5 x 3000 Units
S pack EN-115S по запросу 3000 Units
Recognition Sequence:
5
Heat inactivation: 20 minutes at 80°C.

Ligation and recutting: After 20-fold overdigestion with EcoR V,
>95 % of the DNA fragments can be ligated and recut with this enzyme.

Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration >5 %, or pH >8.0 may result in star activity.

DNA Methylation:
No Inhibition: dam, dcm
Inhibition (Blocked by overlapping): CpG

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (21 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.





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