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Heat inactivation: 20 minutes at 65°C.
DNA Methylation: No Inhibition: dam, EcoKI. Potential Inhibition: dcm. Inhibition (Blocked): CpG.
Unit definition: One unit is the amount of enzyme required to completely digest 1 µg of Ad-2 DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
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