PspP I

Recognition Sequence:

Heat inactivation: 15 minutes at 55°C.

Ligation and recutting: After ten-fold overdigestion with PspP I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.

Note: Incubation at 37°C results in 60 % activity.

DNA Methylation:
No Inhibition: dam
Inhibition (Blocked by overlapping): dcm, CpG

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.