|
Heat inactivation: No.
Ligation and recutting: After ten-fold overdigestion with Sfi I, >90 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation:
No Inhibition: dam
Inhibition (Impaired by overlapping): dcm
Inhibition (Blocked by some combinations of overlapping): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus-2 DNA (3 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
|