SgrB I

Recognition Sequence:

Heat inactivation: 20 minutes at 65°C.

Ligation and recutting: After ten-fold overdigestion with SgrB I,
>98 % of the DNA fragments can be ligated and recut with this enzyme.

Note: Particular sites in λ and ΦX174 DNAs are difficult to cleave with SgrB I, as well as with its prototype Sac II.

DNA Methylation:
No Inhibition: dam, dcm
Inhibition (Blocked): CpG

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 4 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.