Sph I

Recognition Sequence:

Heat inactivation: 20 minutes at 65°C.

Ligation and recutting: After ten-fold overdigestion with Sph I,
>98 % of the DNA fragments can be ligated and recut with this enzyme.

DNA Methylation: No Inhibition: dam, dcm, CpG

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (1 site) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.