qPCR GreenMaster with UNG/highROX

Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible
qPCR GreenMaster with UNG/highROX must be stored in the dark

qPCR GreenMaster with UNG/highROX, 2x conc.
PCR-grade water

qPCR GreenMaster with UNG/highROX contains UNG (Uracil-N-Glycosylase) and 500 nM ROX passive reference dye in the final assay.
UNG and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
The ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.