Store at -20°C in the dark 50x ROX reference dye, 500 µl ROX reference dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the ROX reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 50x concentration.
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