Quality guaranteed for 12 months when stored below 25°C qPCR ProbesMaster Lyophilisate with UNG PCR-grade water qPCR ProbesMaster Lyophilisate with UNG is designed for quantitative real-time analysis of DNA samples using DNA probe based detection. The lyophilisate is recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes. The Master contains all reagents required for qPCR (except template, primer and labeled fluorescent probe). UNG (Uracil-N-Glycosylase) and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
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