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Bcl I Isoschizomers: Fba I, Ksp22 I
5' - T↓GATC A - 3' 3' - A CTAG↑T - 5' |
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Recognition Sequence:
5
Heat inactivation: No.
Ligation and recutting: After ten-fold overdigestion with Bcl I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Large excess of the enzyme results in the appearance of star activity.
DNA Methylation:
No inhibition: dcm, CpG
Inhibition (Blocked): dam
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (dam-; 8 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: No.
Ligation and recutting: After ten-fold overdigestion with Bcl I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Large excess of the enzyme results in the appearance of star activity.
DNA Methylation:
No inhibition: dcm, CpG
Inhibition (Blocked): dam
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (dam-; 8 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-104L | по запросу | 5 x 2500 Units | |
| S pack | EN-104S | по запросу | 2500 Units |
| Документы |
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 Datasheet
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
