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BseB I Isoschizomers: BstN I, BstO I, Bst2U I, Mva I,
Neoschizomers: Ajn I, EcoR II, Psp6 I, PspG I 5' - CC↓W GG - 3' 3' - GG W↑CC - 5' |
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Recognition Sequence:
5
Heat inactivation: No.
Ligation and recutting: After ten-fold overdigestion with BseB I,
<50 % of the DNA fragments can be ligated.
Star activity: Low salt concentration or large excess of enzyme results in the appearance of star activity.
Note: BseB I-cut DNA is difficult to ligate with T4 DNA Ligase. Ligation is enhanced in the presence of 15 % PEG 4000.
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (70 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: No.
Ligation and recutting: After ten-fold overdigestion with BseB I,
<50 % of the DNA fragments can be ligated.
Star activity: Low salt concentration or large excess of enzyme results in the appearance of star activity.
Note: BseB I-cut DNA is difficult to ligate with T4 DNA Ligase. Ligation is enhanced in the presence of 15 % PEG 4000.
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (70 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-108L | по запросу | 5 x 4500 Units | |
| S pack | EN-108S | по запросу | 4500 Units |
| Документы |
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 Datasheet
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
