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BseB I

Isoschizomers: BstN I, BstO I, Bst2U I, Mva I,
Neoschizomers: Ajn I, EcoR II, Psp6 I, PspG I

5' - CC↓W GG - 3'
3' - GG W↑CC - 5'



Recognition Sequence:
5

Heat inactivation: No.

Ligation and recutting: After ten-fold overdigestion with BseB I,
<50 % of the DNA fragments can be ligated.

Star activity: Low salt concentration or large excess of enzyme results in the appearance of star activity.

Note: BseB I-cut DNA is difficult to ligate with T4 DNA Ligase. Ligation is enhanced in the presence of 15 % PEG 4000.

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (70 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.



Название Код Цена Количество
L pack EN-108L по запросу 5 x 4500 Units
S pack EN-108S по запросу 4500 Units


Документы
Datasheet



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