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EcoR I Isoschizomers: Fun II
5' - G↓AATT C - 3' 3' - C TTAA↑G - 5' |
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Recognition Sequence:
5
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After 50-fold overdigestion with EcoR I,
>98 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration >5 %, or pH >8.0 may result in star activity.
DNA Methylation:
No Inhibition: dam, dcm
Inhibition (Impaired by overlapping): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (5 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After 50-fold overdigestion with EcoR I,
>98 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration >5 %, or pH >8.0 may result in star activity.
DNA Methylation:
No Inhibition: dam, dcm
Inhibition (Impaired by overlapping): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (5 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-114L | по запросу | 5 x 15000 Units | |
| S pack | EN-114S | по запросу | 15000 Units |
| Документы |
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 Datasheet
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
