Recognition Sequence: 5
Heat inactivation: 20 minutes at 65°C. Ligation and recutting: After 100-fold overdigestion with Hind III, >98 % of the DNA fragments can be ligated and recut with this enzyme. Star activity: Star activity may be observed in the presence of Mn2+. DNA Methylation: No Inhibition: dam, dcm, CpG Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (7 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
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