| |
Mbo I Isoschizomers: BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 II, NdeI I,
Neoschizomers: BstKT I 5' - ↓GATC - 3' 3' - CTAG↑ - 5' |
 |
Recognition Sequence:
5
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with Mbo I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation:
No Inhibition: dcm
Inhibition (Impaired by overlapping): CpG
Inhibition (Blocked): dam
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (dam-; 116 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with Mbo I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation:
No Inhibition: dcm
Inhibition (Impaired by overlapping): CpG
Inhibition (Blocked): dam
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (dam-; 116 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-120L | по запросу | 5 x 300 Units | |
| S pack | EN-120S | по запросу | 300 Units |
| Документы |
|---|
 Datasheet
|
Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
