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Nco I Isoschizomers: Bsp19 I
5' - C↓CATG G - 3' 3' - G GTAC↑C - 5' |
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Recognition Sequence:
5
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with Nco I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Conditions of high enzyme concentration, or glycerol concentration >5 % may result in star activity.
DNA Methylation: No Inhibition: dam, dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (4 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with Nco I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Conditions of high enzyme concentration, or glycerol concentration >5 % may result in star activity.
DNA Methylation: No Inhibition: dam, dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (4 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-123L | по запросу | 5 x 600 Units | |
| S pack | EN-123S | по запросу | 600 Units |
| Документы |
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 Datasheet
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
