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Nhe I Isoschizomers: AsuNH I,
Neoschizomers: Bmt I 5' - G↓CTAG C - 3' 3' - C GATC↑G - 5' |
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Recognition Sequence:
5
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After 100-fold overdigestion with Nhe I,
>98 % of the DNA fragments can be ligated and recut.
Note: Activity inhibited by salt concentrations > 100mM. Cleaves to leave a 5' CTAG extension which can be efficiently ligated to DNA fragments generated by Avr II, Spe I, or Xba I.
Star activity: Large excess of the enzyme may results in the appearance of star activity.
DNA Methylation:
No Inhibition: dcm, dam
Inhibition (Blocked by some combinations of overlapping): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 1 site) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After 100-fold overdigestion with Nhe I,
>98 % of the DNA fragments can be ligated and recut.
Note: Activity inhibited by salt concentrations > 100mM. Cleaves to leave a 5' CTAG extension which can be efficiently ligated to DNA fragments generated by Avr II, Spe I, or Xba I.
Star activity: Large excess of the enzyme may results in the appearance of star activity.
DNA Methylation:
No Inhibition: dcm, dam
Inhibition (Blocked by some combinations of overlapping): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 1 site) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-146L | по запросу | 5 x 550 Units | |
| S pack | EN-146S | по запросу | 550 Units |
| Документы |
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 Datasheet
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
