1. Каталог >
  2. Реактивы >
  3. Объекты >
  4. Белки >
  5. Ферменты >
  6. Ферменты рестрицикции /модификации >
  7. Рестриктазы >
  8. "N" Ферменты >
  9. Nhe I >

Nhe I

Isoschizomers: AsuNH I,
Neoschizomers: Bmt I

5' - G↓CTAG C - 3'
3' - C GATC↑G - 5'



Recognition Sequence:
5

Heat inactivation: 20 minutes at 65°C.

Ligation and recutting: After 100-fold overdigestion with Nhe I,
>98 % of the DNA fragments can be ligated and recut.

Note: Activity inhibited by salt concentrations > 100mM. Cleaves to leave a 5' CTAG extension which can be efficiently ligated to DNA fragments generated by Avr II, Spe I, or Xba I.

Star activity: Large excess of the enzyme may results in the appearance of star activity.

DNA Methylation:
No Inhibition: dcm, dam
Inhibition (Blocked by some combinations of overlapping): CpG

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 1 site) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.



Название Код Цена Количество
L pack EN-146L по запросу 5 x 550 Units
S pack EN-146S по запросу 550 Units


Документы
Datasheet



Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой