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Not I Isoschizomers: CciN I
5' - GC↓GGCC GC - 3' 3' - CG CCGG↑CG - 5' |
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Recognition Sequence:
5
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After 25-fold overdigestion with Not I,
>98% of the DNA fragments can be ligated and recut with this enzyme.
Note: Supercoiled plasmids may require up to 5-fold more Not I for complete digestion than linear DNA.
DNA Methylation:
No Inhibition: dam, dcm
Inhibition (Blocked): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus-2 DNA (7 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After 25-fold overdigestion with Not I,
>98% of the DNA fragments can be ligated and recut with this enzyme.
Note: Supercoiled plasmids may require up to 5-fold more Not I for complete digestion than linear DNA.
DNA Methylation:
No Inhibition: dam, dcm
Inhibition (Blocked): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus-2 DNA (7 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-124L | по запросу | 5 x 300 Units | |
| S pack | EN-124S | по запросу | 300 Units |
| Документы |
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 Datasheet
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
