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PspP I Isoschizomers: AspS9 I, Cfr13 I, Sau96 I
5' - G↓GNC C - 3' 3' - C CNG↑G - 5' |
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Recognition Sequence:
5
Heat inactivation: 15 minutes at 55°C.
Ligation and recutting: After ten-fold overdigestion with PspP I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
Note: Incubation at 37°C results in 60 % activity.
DNA Methylation:
No Inhibition: dam
Inhibition (Blocked by overlapping): dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: 15 minutes at 55°C.
Ligation and recutting: After ten-fold overdigestion with PspP I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
Note: Incubation at 37°C results in 60 % activity.
DNA Methylation:
No Inhibition: dam
Inhibition (Blocked by overlapping): dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-126L | по запросу | 5 x 900 Units | |
| S pack | EN-126S | по запросу | 900 Units |
| Документы |
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 Datasheet
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
