Recognition Sequence: 5
Heat inactivation: No. Ligation and recutting: After ten-fold overdigestion with Pvu II, >95 % of the DNA fragments can be ligated and recut with this enzyme. Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration >5 %, or pH >8.0 may result in star activity. DNA Methylation: No Inhibition: dam, dcm, CpG Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (15 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
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