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Sfi I

5' - GGCCN NNN↓NGGCC - 3'
3' - CCGGN↑NNN NCCGG - 5'



Recognition Sequence:
5

Heat inactivation: No.

Ligation and recutting: After ten-fold overdigestion with Sfi I, >90 % of the DNA fragments can be ligated and recut with this enzyme.

DNA Methylation:
No Inhibition: dam
Inhibition (Impaired by overlapping): dcm
Inhibition (Blocked by some combinations of overlapping): CpG

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus-2 DNA (3 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.


Название Код Цена Количество
L pack EN-132L по запросу 5 x 400 Units
S pack EN-132S по запросу 400 Units


Документы
Datasheet



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