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SgrB I Isoschizomers: Cfr42 I, Ksp I, Sac II, Sfr303 I
5' - CC GC↓GG - 3' 3' - GG↑CG CC - 5' |
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Recognition Sequence:
5
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with SgrB I,
>98 % of the DNA fragments can be ligated and recut with this enzyme.
Note: Particular sites in λ and ΦX174 DNAs are difficult to cleave with SgrB I, as well as with its prototype Sac II.
DNA Methylation:
No Inhibition: dam, dcm
Inhibition (Blocked): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 4 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with SgrB I,
>98 % of the DNA fragments can be ligated and recut with this enzyme.
Note: Particular sites in λ and ΦX174 DNAs are difficult to cleave with SgrB I, as well as with its prototype Sac II.
DNA Methylation:
No Inhibition: dam, dcm
Inhibition (Blocked): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 4 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-133L | по запросу | 5 x 1600 Units | |
| S pack | EN-133S | по запросу | 1600 Units |
| Документы |
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 Datasheet
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
