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Sph I Isoschizomers: Bbu I, Pae I, SpaH I
5' - G CATG↓C - 3' 3' - C↑GTAC G - 5' |
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Recognition Sequence:
5
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with Sph I,
>98 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation: No Inhibition: dam, dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (1 site) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with Sph I,
>98 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation: No Inhibition: dam, dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (1 site) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-137L | по запросу | 5 x 250 Units | |
| S pack | EN-137S | по запросу | 250 Units |
| Документы |
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 Datasheet
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
