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SseB I Isoschizomers: Aat I, Eco147 I, Pce I, Stu I
5' - AGG↓CCT - 3' 3' - TCC↑GGA - 5' |
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Recognition Sequence:
5
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After 10-fold overdigestion with SseB I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation:
No Inhibition: dam, CpG
Inhibition (Blocked by overlapping): dcm
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 6 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After 10-fold overdigestion with SseB I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation:
No Inhibition: dam, CpG
Inhibition (Blocked by overlapping): dcm
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 6 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-138L | по запросу | 5 x 1500 Units | |
| S pack | EN-138S | по запросу | 1500 Units |
| Документы |
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 Datasheet
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
