ДНК маркеры OZ Biosciences изготавливаются из плазмидной ДНК высокого качества с использованием обедненных по нуклеазам бактерий-хозяев. Перед разделением на аликвоты и лиофилизацией фрагменты ДНК дополнительно очищаются путем депротеинизации и обессоливания. Концентрация фрагментов корректируется так, чтобы каждая пробирка содержала 50 мкг ДНК.
Размер: 50мкг маркер + 1 мл буфер нанесения Хранение: +4°C Условия транспортировки: комнатная температура
ДокументыПрименение
Recommended for: Analysis of mid-sized DNA fragments from restriction analysis and plasmid vectors in the average size range. Features: 10 fragments with increments of 100bp with an additional 150bp fragment for the analysis of 100-200bp PCR-products. Two times concentrated 500bp-band for easy detection. Others bands are equimolar concentration (1:1 ratio). Needs 70-80mm distance in 1.2% - 1.5% agarose gels for complete separation of bands.
Recommended for: Analysis of mid- and large-sized DNA fragments from restriction analysis and plasmid vectors in the average size range.Features: In addition to the above mentioned 100bp-DNA ladder (60%), 40% of the total DNA-mass is an equimolar mixture of 6 high-molecular DNA fragments ranging from 1500 to 5000bp. On 1.0% - 1.5% agarose gels, the extended ladder looks like two separated, set-on-top DNA ladders.
Recommended for: Analysis of large DNA fragments and PCR-products. Features: 11 fragments with increments of 2000, 1000 or 500bp. All bands are in equimolar concentration. Needs 70-800mm distance in 1.0% - 1.2% agarose gels for complete separation of bands.
Recommended for: Quick gel separation in high throughput applications. Features: 5 fragments with 500bp increments. Needs 1.5 - 2.5 mm distance in 1.2% - 1.5% agarose gels for complete separation of bands.
Recommended for: Quick gel separation in high throughput applications. Analysis of mid-and small-sized DNA fragments and PCR products. Features: Easily detectable double bands of 2176 / 1766bp and 1230 / 1033bp. Used on 1.2% - 1.5% agarose gels with fast spread-up of the fragments.
Recommended for: Analysis of small PCR products. Features: Used on 1.2% - 1.5% agarose gels with fast spread-up of the fragments.
Recommended for: Gel analysis on 1.8% - 2.2% agarose gels. Features: Large number of fragments in the low molecular range; mainly clustered in three areas of 3-4 fragments. Contains also very small fragments, which take longer for complete separation.
Recommended for: Gel analysis on 1.8% - 2.2% agarose gels. Wide range of fragment sizes. Features: Fast spread-up of the fragments on 1.2% - 1.5% agarose gels
Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой |
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