Анализ экспрессии генов |
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β-Gal Assay Kits provide convenient, accurate quantitation of β-Gal expression.β-Galactosidase Assay Kit: Red (CPRG) Highly sensitive, uses the red substrate chlorophenol red-β-D-galactopyranoside (CPRG) to determine β-gal expression levels. The CPRG kit is ideal for conditions in which β-galactosidse expression levels are low.
β-Galactosidase Assay Kit: Yellow (ONPG) Measures development of the bright yellow substrate o-nitrophenyl-β-D-galactopyranoside (ONPG). The ONPG kit provides a quick method for quantifying β-galactosidase expression in cultured cells.
X-Gal Staining Assay Kit: Designed for sensitive and low-background visualization of cultured cells or tissues containing cells carrying the lacZ gene that codes for β-galactosidase enzyme. Cells transfected with a β-gal-expressing plasmid appear blue* under the microscope.
*The β-galactosidase enzyme catalyzes the hydrolysis of X-gal (5-bromo-3-indoyl-β-D- galactopyranoside) yielding 5-bromo-4-chloro-3-hydroxyindole which spontaneously converts into 5,5'-dibromo-4,4'-dichloro-indigo, an intensely blue insoluble product.
Параметры
β-Galactosidase Assay Kit: Red (CPRG)
- 55 ml each of 5x Lysis Buffer, Standard Dilution Buffer, Substrate Buffer & Stop Buffer
- 10x CPRG Substrate Stock (Chlorophenol red-β-D-galactopyranoside) (5 vials of 1 ml)
- β-galactosidase enzyme standard, 40 units (100 ml)
- Shipped frozen: Store CPRG substrate at -20ºC, all other components at 4ºC (Stable 6 months when stored properly)
β-Galactosidase Assay Kit: Yellow (ONPG)
- 55 ml each of 5x Lysis Buffer, Standard Dilution Buffer, Substrate Buffer, Stop Buffer & ONPG Substrate Solution & 3mg/ml (o-nitrophenyl-β-D-galactopyranoside)
- β-galactosidase enzyme standard, 40 units (100 ml)
- Shipped frozen: Store β-galactosidase enzyme standard at -20ºC, all other components at 4ºC (Stable 6 months when stored properly)
X-Gal Staining Assay Kit
- 125 ml each of Fixing Buffer & Staining Buffer
- 10X PBS (75 ml)
- 25x X-Gal stock (4 vials of 1 ml)
Документы
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