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Клетки гладкой мускулатуры аорты человека: HAOSMC. Первичные клеточные линии Cell Applications.

Human Aortic Smooth Muscle Cells: HAOSMC

Клетки Гладкой Мускулатуры Аорты Человека (HAOSMC) предоставляют прекрасную модельную систему для изучения всех аспектов сердечно-сосудистый функций и заболеваний, особенно связанных с механизмами гиперплазии и гипертрофии клеток гладкой мускулатуры интимы, ведущих к закупорке сосудов при атеросклерозе и рестенозе стента.

HAOSMC от Cell Applications, Inc. были использованы в десятках исследований, например:

  • Сигнальные пути цитокинов и фактора роста вовлечены в молекулярную регуляцию пролиферации гладкомышечных клеток, их миграцию, а также сосудистые функции в целом
  • Изучение связанного с гипергликемией фактора риска атеросклероза у больных диабетом, как и эффектов энанола на кальцификацию сосудов
  • Отложение внеклеточного матрикса и его роль в здоровье сердца и сосудов, восстановление поврежденных сосудов и успешная инженерия тканей.
  • Механизмы и эффекты механорегуляции на пролиферацию и функционирование клеток гладкой мускулатуры
  • Усовершенствованная технология стента, в том числе новые поверхностные материалы
  • Системы доставки лекарств и генов
  • Рестенозы и другие закупоривающие патологии сосудов

Характеристика: положительно для специфичной для клеток гладкой мускулатуры альфа-актиновой экспрессии

Параметры

Tissue:
Human tunica intima and tunica media of normal human, fibrous plaque-free aorta.  Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HAOEC(354-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
Shipped in Smooth Muscle Cell Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings


Документы



Публикации

2016

Oros, M. 2016. Ethanol accelerates vascular calcification in human aortic smooth muscle cells: Quantitative trait locus mapping reveals alcohol consumption genes. PhD Thesis, University of Debrecen, Doctoral School of Molecular and Immune Biology.

2015

Helkin, A., K. Maier, and V. Gahtan. 2015. Thrombospondin-1, -2 and -5 have differential effects on vascular smooth muscle cell physiology. BBRC, doi:10.1016/j.bbrc.2015.07.044.
2014
Seymour, K., J. Stein, X. Han, K. Maier, and V. Gahtan. 2014. Statins and Nitric Oxide Donors Affect Thrombospondin 1-Induced Chemotaxis. Vasc Endovascular Surg, 48:470-475.
Venkataraman, L., B. Sivaraman, P. Vaidya, and A. Ramamurthi. 2014. Nanoparticulate delivery of agents for induced elastogenesis in three-dimensional collagenous matrices. Journal of tissue engineering and regenerative medicine:10.1002/term.1889.
2013
Almontashiri, N.A.M., M. Fan, B.L.M. Cheng, H.-H. Chen, R. Roberts, and A.F.R. Stewart. 2013. Interferon-γ Activates Expression of p15 and p16 Regardless of 9p21.3 Coronary Artery Disease Risk Genotype. Journal of the American College of Cardiology. 61:143-147.
Deshpande, D., D.R. Janero, and M. Amiji. 2013. Engineering of an ω-3 polyunsaturated fatty acid-containing nanoemulsion system for combination C6-ceramide and 17β-estradiol delivery and bioactivity in human vascular endothelial and smooth muscle cells. Nanomedicine: Nanotechnology, Biology and Medicine. 9:885-894.
Hirase, T., H. Hara, Y. Miyazaki, N. Ide, A. Nishimoto-Hazuku, H. Fujimoto, C.J. Saris, H. Yoshida, and K. Node. 2013. Interleukin 27 inhibits atherosclerosis via immunoregulation of macrophages in mice. American Journal of Physiology-Heart and Circulatory Physiology. 305:H420-429.
Kakade, S., and G. Mani. 2013. A comparative study of the effects of vitamin C, sirolimus, and paclitaxel on the growth of endothelial and smooth muscle cells for cardiovascular medical device applications. Drug design, development and therapy. 7:529.
Lamichhane, S., S. Lancaster, E. Thiruppathi, and G. Mani. 2013. Interaction of Endothelial and Smooth Muscle Cells with Cobalt–Chromium Alloy Surfaces Coated with Paclitaxel Deposited Self-Assembled Monolayers. Langmuir. 29:14254-14264.
Gleason. 2013. Mechanism of aortic medial matrix remodeling is distinct in patients with bicuspid aortic valve. The Journal of thoracic and cardiovascular surgery: doi: 10.1016/j.jtcvs.2013.1004.1028.
Stein, J., C. Iwuchukwu, K. Maier, and V. Gahtan. 2013. Thrombospondin-1-induced smooth muscle cell chemotaxis and proliferation are dependent on transforming growth factor-β2 and hyaluronic acid synthase. Molecular and cellular biochemistry. 384:181-186.
Venkataraman, L., C.A. Bashur, and A. Ramamurthi. 2013. Impact of Cyclic Stretch on Induced Elastogenesis within Collagenous Conduits. Tissue Engineering Part A: doi:10.1089/ten.TEA.2013.0294.
2012
Acilan, C., M. Serhatli, O. Kacar, Z. Adiguzel, A. Tuncer, M. Hayran, and K. Baysal. 2012. Smooth Muscle Cells Isolated from Thoracic Aortic Aneurysms Exhibit Increased Genomic Damage, but Similar Tendency for Apoptosis. DNA Cell Biol, 31:1523-1534.
Almontashiri, N. 2012. A gain of function variant of the mitochondrial matrix protease SPG7 is associated with increased risk of coronary artery disease. Vol. MSc dissertation. University of Ottawa.
Oros, M., E. Zavaczki, C. Vadasz, V. Jeney, A. Tosaki, I. Lekli, G. Balla, L. Nagy, and J. Balla. 2012. Ethanol increases phosphatemediated mineralization and osteoblastic transformation of vascular smooth muscle cells. Journal of cellular and molecular medicine. 16:2219-2226.
Santiago, F.S. 2012. Regulatory Mechanisms in Vascular Injury and Repair. University of New South Wales, PhD dissertation.
Venkataraman, L. 2012. Induced elastic matrix synthesis within 3-dimensional collagen constructs. PhD dissertation, Clemson University.
2011
Chen, J., M.Y. Liu, C.R. Parish, B.H. Chong, and L. Khachigian. 2011. Nuclear import of early growth response-1 involves importin-7 and the novel nuclear localization signal serine-proline-serine. Intern. J. of biochemistry & cell biology. 43:905-912.
Gacchina, C., T. Brothers, and A. Ramamurthi. 2011. Evaluating smooth muscle cells from CaCl2-induced rat aortal expansions as a surrogate culture model for study of elastogenic induction of human aneurysmal cells. Tissue Engineering Part A. 17:1945-1958.
Liu, X., E.-Z. Jin, J.-X. Zhi, and X.-Q. Li. 2011. Identification of HZF1 as a novel target gene of the MEF2 transcription factor. Molecular medicine reports. 4:465-469.
2010
Brito, L.A., S. Chandrasekhar, S.R. Little, and M.M. Amiji. 2010. In vitro and In vivo studies of local arterial gene delivery and transfection using lipopolyplexesembedded stents. Journal of Biomedical Materials Research Part A. 93:325-336.
Gacchina, C.E. 2010. Cell-mediated elastic matrix regeneration toward regression of abdominal aortic aneurysms. PhD Dissertation, Clemson University.
Seymour, K., X. Han, B. Sadowitz, K.G. Maier, and V. Gahtan. 2010. Differential effect of nitric oxide on thrombospondin-1-, PDGF- and fibronectin-induced migration of vascular smooth muscle cells. The American J. Surgery. 200:615-619.
2009
Argraves, W., A. Tanaka, E. Smith, W. Twal, K. Argraves, D. Fan, and C. Haudenschild. 2009. Fibulin-1 and fibrinogen in human atherosclerotic lesions. Histochemistry and Cell Biology, 132:559-565.
Brito, L. 2009. Targeted endothelial nitric oxide synthase gene delivery with lipopolyplexes for the treatment of coronary restenosis. ProQuest.
Fischer, L.J., S. McIlhenny, T. Tulenko, N. Golesorkhi, P. Zhang, R. Larson, J. Lombardi, I. Shapiro, and P.J. DiMuzio. 2009. Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force. Journal of Surgical Research. 152:157-166.
Hampson, A. 2009. Transcriptional Regulation of Soluble Guanylyl Cyclase. B.Sc. honors thesis, Dublin Institute of Technology.
Jiang, X., P.F. Austin, R.A. Niederhoff, S.R. Manson, J.J. Riehm, B.L. Cook, G. Pengue, K. Chitaley, K. Nakayama, and K.I. Nakayama. 2009. Mechanoregulation of proliferation. Molecular and cellular biology. 29:5104-5114.
Liu, M.Y., and L.M. Khachigian. 2009. HDAC-1 is Enriched at the PDGF-D Promoter in Response to IL-1beta and Forms a Cytokine-Inducible Gene Silencing Complex with NF-kappaB p65 and IRF-1. Journal of Biological Chemistry:10.1074/jbc.M1109.061903.
Morrow, D., J.P. Cullen, W. Liu, S. Guha, C. Sweeney, Y.A. Birney, N. Collins, D. Walls, E.M. Redmond, and P.A. Cahill. 2009. Sonic Hedgehog induces Notch target gene expression in vascular smooth muscle cells via VEGF-A. Arterioscler Thromb Vasc Biol. 29:1112-1118.
2008
Brito, L., S. Little, R. Langer, and M. Amiji. 2008. Poly(β-amino ester) and Cationic Phospholipid-Based Lipopolyplexes for Gene Delivery and Transfection in Human Aortic Endothelial and Smooth Muscle Cells. Biomacromolecules. 9:1179-1187. HAOSMC
2007
Srinivas, A., and A. Ramamurthi. 2007. Effects of gamma-irradiation on physical and biologic properties of crosslinked hyaluronan tissue engineering scaffolds. Tissue engineering. 13:447-459.
Wang, M., J. Zhang, L. Jiang, G. Spinetti, G. Pintus, R. Monticone, F. Kolodgie, R. Virmani, and E. Lakatta. 2007. Proinflammatory Profile Within the Grossly Normal Aged Human Aortic Wall. Hypertension, 50:219-227.
2006
Leik, C.E., E.J. Su, P. Nambi, D.L. Crandall, and D.A. Lawrence. 2006. Effect of pharmacologic plasminogen activator inhibitor-1 inhibition on cell motility and tumor angiogenesis. Journal of Thrombosis and Haemostasis. 4:2710-2715.
Scheller, A. 2006. Notch signaling pathway in human vascular smooth muscle cell differentiation. Dublin City University, M.Sc. dissertation.
2005
Gravel, S.-P., and M.J. Servant. 2005. Roles of an IκB Kinase-related Pathway in Human Cytomegalovirus-infected Vascular Smooth Muscle Cells: A MOLECULAR LINK IN PATHOGEN-INDUCED PROATHEROSCLEROTIC CONDITIONS. Journal of Biological Chemistry. 280:7477-7486.
Sottile, J., and J. Chandler. 2005. Fibronectin Matrix Turnover Occurs through a Caveolin-1–dependent Process. Molecular biology of the cell. 16:757-768.
Toyoshima, T., N. Nishi, H. Kusama, R. Kobayashi, and T. Itano. 2005. 36-kDa microfibril-associated glycoprotein (MAGP-36) is an elastin-binding protein increased in chick aortae during development and growth. Exp. cell research. 307:224-230.
2002
Sottile, J., and D.C. Hocking. 2002. Fibronectin Polymerization Regulates the Composition and Stability of Extracellular Matrix Fibrils and Cell-Matrix Adhesions. Molecular biology of the cell. 13:3546-3559.
1999
Wang, R., M. Xu, R. Marcel, G. Bouliane, and D.Z. Fisher. 1999. Selective neointimal gene transfer in an avian model of vascular injury. Atherosclerosis. 146:71-82.

 

1998

 

Leung, K.H., V. Pippalla, A. Kreutter, and M. Chandler. 1998. Functional effects of FGF-13 on human lung fibroblasts, dermal microvascular endothelial cells, and aortic smooth muscle cells. Biochem. & Biophys. Research Communic. 250:137-142.

 




НазваниеКодЦена
HAOSMC Total Kit: Media, Subculture Reagents & Cells, Adult 354K-05a по запросу
HAOSMC Total Kit: Media, Subculture Reagents & Cells, Fetal 354K-05f по запросу
HAOSMC Total Kit: Media, Subculture Reagents & Cells, Pooled, Adult 354pK-05a по запросу



НазваниеКодЦена
Cryopreserved HAOSMC 354-05a по запросу



НазваниеКодЦена
Cryopreserved HAOSMC 354p-05a по запросу



НазваниеКодЦена
Cryopreserved HAOSMC 354-05f по запросу



НазваниеКодЦена
Cryopreserved HAOSMC from donor with Type 2 Diabetes 354T2D-05a по запросу



НазваниеКодЦена
HAOSMC-T2D Total Kit: Media, Subculture Reagents & Cells, from donor with Type 2 Diabetes, Adult 354T2DK-05a по запросу



НазваниеКодЦена
125 x 24-Well TF350K по запросу
25 x 24-Well Rxns TF350KS по запросу



НазваниеКодЦена
Total RNA prepared from human heart tissue 1H30-50 по запросу
Total RNA prepared from human heart tissue 1H30-250 по запросу



НазваниеКодЦена
Proliferating HAOSMC 355-25a по запросу
Proliferating HAOSMC 355-75a по запросу
Proliferating HAOSMC 355-6Wa по запросу
Proliferating HAOSMC 355-96Wa по запросу



НазваниеКодЦена
Proliferating HAOSMC 355-25f по запросу
Proliferating HAOSMC 355-75f по запросу
Proliferating HAOSMC 355-6Wf по запросу
Proliferating HAOSMC 355-96Wf по запросу



НазваниеКодЦена
Basal medium (contains no growth supplement).В  Add GS before use. 310-500 по запросу



НазваниеКодЦена
Promotes cells to change from one type to another, more specialized 311D-250 по запросу



НазваниеКодЦена
Added to Basal Medium to create Growth Medium 311-GS по запросу



НазваниеКодЦена
100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 090K по запросу



Extended Family Products

НазваниеКодЦена
100 tests 028-S по запросу
500 tests 028-01 по запросу



НазваниеКодЦена
Total RNA prepared from Human Aortic Smooth Muscle Cells, adult 354-R10a по запросу
Total RNA prepared from Human Aortic Smooth Muscle Cells, adult 354-R25a по запросу



Related Products

НазваниеКодЦена
All-in-one ready-to-use 311-500 по запросу



НазваниеКодЦена
Basal medium & growth supplement sold together packaged separately 311K-500 по запросу




Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой