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Клетки эндотелия легочной артерии человека: HPAEC. Первичные клеточные линии Cell Applications. Human Pulmonary Artery Endothelial Cells: HPAEC

Клетки Эндотелия Легочной Артерии (HPAEC) предоставляют прекрасную модельную систему для изучения многих аспектов сердечно-сосудистых функций и заболеваний, включая связанные с диабетом расстройства, механизмы эндотелиальной дисфункции, вызыванные условиями окружающей среды, оксидативным стрессом, гипоксией и воспалением, а также способов действия различных природных соединений и потенциальных лекарств.

HPAEC от Cell Applications использовались для:

• Investigate vasculoprotective effects of propofol and show tat it induces Angiotensin-converting enzyme 2(ACE2) transcription via a PI3K-dependent mechanism, demonstrating its potential for treating pulmonary arterial hypertension
• Analyze hits from a high-throughput screening assay and discover anziaic acid, from the lichen Hypotrachyna sp., as novel inhibitor for both Y. pestis and E. coli topoisomerase I
• Show that endothelin-1 that is released by endothelial cells during acute crisis in sickle cell anemia, increases vascular constriction
• Demonstrate that pollutant particles cause vascular dysfunction by up-regulating clotting-related genes, such as F2RL2, FOS, JUN, NF-kB, F2RL2, HMOX1 and tissue factor F3-mediated increase in IL-6 and IL-8 and activation of NADPH oxidase
• Demonstrate the central role of AMP-activated protein kinase (AMPK) in normal endothelial barrier function that is disrupted by exposure to lipopolysaccharides inhibiting AMPK, and causing endothelial hyperpermeability and lung injury
• Confirm anti-inflammatory and cardioprotective effects of resveratrol by showing that it suppresses IL-13 and TNF-α induced eotaxin-1 gene expression, inhibits expression of JAK-1, reduces STAT6 phosphorylation and decreases p65 subunit of NF-κB
• Determine that cGMP can prevent oxidant-induced damage to the endothelial barrier function by activating cGMP-dependent protein kinase PKGI through a Ca2+ independent mechanism
• Demonstrate that exposure to radiation causes endothelial cells senescence (as judged by induction, downstream of mTOR, of senescence markers p53, p21/waf1, and senescence-associated b-Gal) due to up-regulation of proliferative signaling (IGF-1 and IGF-2 and hyperphosphorylation of IGF-1R) in the presence of cell cycle arrest
• Show that vanadium exposure causes pulmonary vasoconstriction mediated in part by the inhibition of endothelial NO production via PKC-dependent phosphorylation of Thr495 of eNOS
• Evaluate, along with Human Dermal Microvascular Endothelial Cells (CADMEC) and Human Umbilical Cord Vascular Endothelial Cells (HUVEC), all obtained от Cell Applications, Inc., the cytotoxic effects of a novel pore-forming protein, proposed as an anti-tumor agent
• Compare effects of BMP-4 on HPAEC and Human Coronary Artery Endothelial Cells (HCAEC, also от Cell Applications, Inc.) and showing that only in HCAEC BMP-4 treatment induced ROS, activated NF-kB, ICAM-1 and increased monocyte adhesiveness, explaining why its upregulation leads to atherosclerosis and hypertension in the systemic, but not pulmonary circulation
• Demonstrate that macro- , but not micro-vascular pulmonary endothelial cells accumulate HIF-1 under hypoxic conditions
• Confirm SPNS2 role as a transporter for S1P, a bioactive lipid, by monitoring S1P release from HPAEC and Human Umbilical Vein Endothelial Cells, both obtained от Cell Applications, Inc., and by showing that S1P was not released when SPNS2 was silenced with siRNA. Additionally, quantitative real-time PCR indicated that Spns2 mRNA was transcribed at higher levels in venous HUVEC compared to arterial HPAEC, suggesting heterogeneity in the expression level of SPNS2 among different vascular beds.

Обратите внимание, что некоторые публикации описывают разницу между эндотелиальными клетками из разных сосудистых русел и между макро- и микрососудистыми клетками из одного органа, подчеркивая важность подтверждения каких-либо новых результатов, полученных на клеточных лотах, полученных из других источников. Cell Applications, Inc. предлагает широчайшее разнообразие эндотелиальных клеток и хорошо подготовлены для  удовлетворения этой потребности.

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Эндотелий: клетки