Фибробласты легких человека: HLF. Первичные клеточные линии Cell Applications.

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Фибробласты легких человека: HLF. Первичные клеточные линии Cell Applications. Human Lung Fibroblasts: HLF

Фибробласты Легких Человека (HLF) обеспечивают прекрасную модель для изучения многих аспектов функций и патологий человеческих легких.

HLF от Cell Applications, Inc. были использованы во множестве исследований, например для демонстрации:

(Текст на языке оригинала)

Differential gene expression, cytokines, growth factors and interleukins
Receptor activation, inhibition and receptor-interacting protein
Cell behavior changes, including synthesis of collagen and other extracellular matrix molecules, as well as actin reorganization, collagen contraction and fibroblast / myofibroblast trans-differentiation.
Lung disease, disorders and injury, including hypoxia, tissue rigidity, pulmonary fibrosis and hypertension
That unwanted cell growth can be inhibited, and that the healing process can be understood and improved

Параметры

Tissue:	Normal human lung parenchyma. Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:	1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:	Ampoule of cryopreserved HLF (506-05a or 506-05f), 500 ml of Fibroblast Growth Medium (116-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:	Shipped in Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:	Can be cultured at least 12 doublings.

Документы

Публикации

2015

Peng, H., W. Gao, F. Chong, H. Liu, and J. Zhang. 2015. Semaphorin 4A enhances lung fibrosis through activation of Akt via PlexinD1 receptor. J Biosci, DOI 10.1007/s12038-015-9566-9.

2014

Nyp, M., A. Navarro, M. Rezaiekhaligh, R. Perez, S. Mabry, and I. Ekekezie. 2014. TRIP-1 via AKT modulation drives lung fibroblast/myofibroblast trans-differentiation. Respiratory Research. 15:19.

2013

Ni, J., Z. Dong, W. Han, D. Kondrikov, and Y. Su. 2013. The role of RhoA and cytoskeleton in myofibroblast transformation in hyperoxic lung fibrosis. Free Radical Biology and Medicine. 61:26-39.

2011

Buck, M., and M. Chojkier. 2011. C/EBPβ-Thr217 Phosphorylation Signaling Contributes to the Development of Lung Injury and Fibrosis in Mice. PloS one. 6:e25497.

Zheng, M., D.M. Jones, C. Horzempa, A. Prasad, and P.J. McKeown-Longo. 2011. The first type III domain of fibronectin is associated with the expression of cytokines within the lung tumor microenvironment. Journal of Cancer. 2:478.

2009

Navarro, A., M. Rezaiekhaligh, J.A. Keightley, S.M. Mabry, R.E. Perez, and I.I. Ekekezie. 2009. Higher TRIP-1 level explains diminished collagen contraction ability of fetal versus adult fibroblasts. American Journal of Physiology - Lung Cellular and Molecular Physiology. 296:L928-L935.

Okada, M., K. Suzuki, M. Matsumoto, K. Takada, T. Nakanishi, H. Horikoshi, T. Higuchi, Y. Hosono, M. Nakayama, and F. Ohsuzu. 2009. Effects of angiotensin on the expression of fibrosis-associated cytokines, growth factors, and matrix proteins in human lung fibroblasts. Journal of Clinical Pharmacy and Therapeutics. 34:289-299.

Tofovic, S.P., X. Zhang, E.K. Jackson, H. Zhu, and G. Petrusevska. 2009. 2-methoxyestradiol attenuates bleomycin-induced pulmonary hypertension and fibrosis in estrogen-deficient rats. Vascular pharmacology. 51:190-197.

2008

Tofovic, S.P., X. Zhang, H. Zhu, E.K. Jackson, O. Rafikova, and G. Petrusevska. 2008. 2-Ethoxyestradiol is antimitogenic and attenuates monocrotaline-induced pulmonary hypertension and vascular remodeling. Vascular pharmac. 48:174-183.

2003

Ikeda, J.,, Y. Okada, H., Sakai, and H. Osuga. 2003. Anti-neurodegenerative agents. Patent Applications US 20050261306 A1.

1998

Leung, K.H., V. Pippalla, A. Kreutter, and M. Chandler. 1998. Functional effects of FGF-13 on human lung fibroblasts, dermal microvascular endothelial cells, and aortic smooth muscle cells. Biochem. & Biophys. Research Communic. 250:137-142.

Cryopreserved HLF Total Kit

Название	Код	Цена
HLF Total Kit: Media, Subculture Reagents & Cells, Adult	506K-05a	по запросу
HLF Total Kit: Media, Subculture Reagents & Cells, Fetal	506K-05f	по запросу

Cryopreserved HLF, adult

Название	Код	Цена
Cryopreserved HLF	506-05a	по запросу

Cryopreserved HLF, fetal

Название	Код	Цена
Cryopreserved HLF	506-05f	по запросу

FGF9 ELISA Kit, Human

Название	Код	Цена
Human FGF9 ELISA Kit	CL0348	по запросу

Fibroblast Basal Medium

Название	Код	Цена
Basal medium (contains no growth supplement).В Add GS before use.	115-500	по запросу

Fibroblast Growth Medium Kit

Название	Код	Цена
Basal medium & growth supplement sold together packaged separately	116K-500	по запросу

Fibroblast Growth Supplement

Название	Код	Цена
Added to Basal Medium to create Growth Medium	116-GS	по запросу

Lung RNA

Название	Код	Цена
Total RNA prepared from human lung tissue	1H40-50	по запросу
Total RNA prepared from human lung tissue	1H40-250	по запросу

Proliferating HLF, adult

Название	Код	Цена
Proliferating HLF	507-96Wa	по запросу
Proliferating HLF	507-25a	по запросу
Proliferating HLF	507-75a	по запросу
Proliferating HLF	507-6Wa	по запросу

Proliferating HLF, fetal

Название	Код	Цена
Proliferating HLF	507-25f	по запросу
Proliferating HLF	507-75f	по запросу
Proliferating HLF	507-96Wf	по запросу
Proliferating HLF	507-6Wf	по запросу

Subculture Reagent Kit

Название	Код	Цена
100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	090K	по запросу

Extended Family Products

Cyto-X Colorimetric Cell Counting Reagent

Название	Код	Цена
100 tests	028-S	по запросу
500 tests	028-01	по запросу

Cytofect™ Fibroblast Transfection Kit

Название	Код	Цена
25 x 24-Well Rxns	TF103KS	по запросу
250 x 24-Well Rxns	TF103K	по запросу

FGF-acidic

Название	Код	Цена
Human Fibroblast Growth Factor acidic	RP1029-50	по запросу
Human Fibroblast Growth Factor acidic	RP1029-10	по запросу
Human Fibroblast Growth Factor acidic	RP1029-1000	по запросу

HLF RNA

Название	Код	Цена
Total RNA prepared from Human Lung Fibroblasts, fetal	506-R10f	по запросу
Total RNA prepared from Human Lung Fibroblasts, fetal	506-R25f	по запросу