Остеобласты человека: HOb. Первичные клеточные линии Cell Applications.

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Остеобласты человека: HOb. Первичные клеточные линии Cell Applications. Human Osteoblasts: HOb

Остеобласты Человека (HOb) от Cell Applications, Inc. выделены из эмбриональных или взрослых человеческих костей. Клетки, положительные на минерализацию кости, обеспечивают прекрасную модельную систему для исследований, связанных со скелетной системой. Кроме того, доступны остеобласты от доноров с остеоартритом или ревматоидным артритом.

Клетки HOb были использованы для изучения:

Действия, сигнальных путей и взаимодействия факторов роста, цитокинов и интерлейкинов.
Ядерных факторов и генной экспрессии.
Клеточной пролиферации и дифференциации.
Формировании кости, морфогенеза и костных морфогенных белков.
Механизмов, стоящих за костными нарушениями и дефектами, включая потерю костной массы, остеопорозы, ингибирование остеокластов, периодонтит, артриты и связанную с ними продукцию металлопротеиназ.
Онкология, например, остеосаркома и онкогены. Для фармацевтической индустрии клетки играли ключевую роль в разработке.
Новые фармацевтические стратегии лечения и методы доставки лекарств.
Также свою роль исследования на остеобластах сыграли в разработке ортопедических имплантов и тканевой инженерии.

Параметры

Tissue:	Normal human bone. Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:	2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:	Ampoule of cryopreserved Human Osteoblasts (406-05), 500 ml of Human Osteoblast Growth Medium (417-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:	Shipped in Osteoblast Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:	Can be cultured at least 10 doublings

Документы

Публикации

2016

Fradique, R., T. Correia, S. Miguel, K. de Sá, D. Figueira, A. Mendonça and I. Correia. 2016. Production of new 3D scaffolds for bone tissue regeneration by rapid prototyping. Journal of Materials Science: Materials in Medicine, 10.1007/s10856-016-5681-x.

Junkar, I., M. Kulkarni, B. Drašlerc, N. Rugelj, A. Mazare, A. Flaškerb, D. Drobne, P. Humpoličeke, M. Resnik, P. Schmuki, M. Mozetiča and A. Igličb. Influence of various sterilization procedures on TiO₂ nanotubes used for biomedical devices. Bioelectrochemistry, doi:10.1016/j.bioelechem.2016.02.001.

Kim, G., O. Elnabawi, D. Shin and E. Pae. 2016. Transient Intermittent Hypoxia Exposure Disrupts Neonatal Bone Strength. Frontiers in Pediatrics, DOI=10.3389/fped.2016.00015.

Ueda, M., T. Goto, K. Kuroishi, K. Gunjigake, E. Ikeda, S. Kataoka, M. Nakatomi, T. Toyono, Y. Seta, and T. Kawamoto. 2016. Asporin in compressed periodontal ligament cells inhibits bone formation. Archives of Oral Biology, 62:86-92.

Ueda, M., K. Kuroishi, K. Gunjigake, E. Ikeda and T. Kawamoto. 2016. Expression of SOST/sclerostin in compressed periodontal ligament cells. Journal of Dental Sciences, doi:10.1016/j.jds.2016.02.006.

Wu, R., W. Wang, G. Huang, X. Mao, Y. Chen, Q. Tang and L. Liao. 2016. Endothelin‑1 induces oncostatin M expression in osteoarthritis osteoblasts by trans‑activating the oncostatin M gene promoter via Ets‑1. Molecular Medicine Reports, 13:3559-3566.

2015

Padilla, S., A. de Castro, A. Gar Garzón-Gutiérrez, L. Benito, S. Enciso, M. Canillas, and R. Carrodeguas. 2015. Novel Nanostructured Zn-substituted Monetite Based Biomaterial for Bone Regeneration. J Nanomed Nanotechnol, 2015, 6:5, http://dx.doi.org/10.4172/2157-7439.1000325.

2014

Both, J., O. Krijgsman, J. Bras, G. Schaap, F. Baas, B. Ylstra, and T. Hulsebos. 2014. Focal Chromosomal Copy Number Aberrations Identify CMTM8 and GPR177 as New Candidate Driver Genes in Osteosarcoma. PLoS ONE 9(12): e115835.

Frandsen, C., K. Brammer, K. Noh, G. Johnston, and Sungho Jin. 2014. Tantalum coating on TiO 2 nanotubes induces superior rate of matrix mineralization and osteofunctionality in human osteoblasts. Materials Science and Engineering, 37:332–341.

Lee, Y., H. Ji, S. Lee, S. Hong, H Yang, M. Yoo1 and K. Kim. 2014. The role of adiponectin in the production of IL-6, IL-8, VEGF and MMPs in human endothelial cells and osteoblasts: implications for arthritic joints. Experimental & Molecular Medicine 46, online 17 January, e72.

Mao, Y.-W., R.-D. Lin, H.-C. Hung, and M.-H. Lee. 2014. Stimulation of Osteogenic Activity in Human Osteoblast Cells by Edible Uraria crinita. J Agric Food Chem, 62:5581–5588.

Pilia, M., T. Guda, B. Pollot, V. Aguera, and M. Appleford. 2014. Local microarchitecture affects mechanical properties of deposited extracellular matrix for osteonal regeneration. Materials Science and Engineering: C, 35:122-133.

2013

Kwak, J.-H., S.-R. Lee, H.-J. Park, H.-E. Byun, E.-H. Sohn, B.-O. Kim, D.-K. Rhee, and S. Pyo. 2013. Kobophenol A enhances proliferation of human osteoblast-like cells with activation of the p38 pathway. Int. Immunopharm. 17:704-713.

Pilia, M., T. Guda, B. Pollot, V. Aguero, and M. Appleford. 2013a. Local microarchitecture affects mechanical properties of deposited extracellular matrix for osteonal regeneration. Materials Science and Engineering: C. 35:122-133.

Pilia, M., T. Guda, S.M. Shiels, and M.R. Appleford. 2013b. Influence of substrate curvature on osteoblast orientation and extracellular matrix deposition. Journal of biological engineering. 7:23.

2012

Appleford, M. and M. Pilia. 2012. Cortical bone scaffold for guided osteon regeneration in load-bearing orthopaedic applications. Patent Application US 20140236312 A1.

Both, J., T. Wu, J. Bras, G.R. Schaap, F. Baas, and T.J. Hulsebos. 2012. Identification of Novel Candidate Oncogenes in Chromosome Region 17p11. 2-p12 in Human Osteosarcoma. PloS one. 7:e30907.

Frandsen, C. 2012. An Extensive Analysis of Modified Nanotube Surfaces for Next-Generation Orthopedic Implants. PhD Dissertation, UCSD.

Ni, J., C.J. Frandsen, K. Noh, G.W. Johnston, G. He, T. Tang, and S. Jin. 2012. Fabrication of thin film TiO2 nanotube arrays on Co-28Cr-6Mo alloy by anodization. Materials Science and Engineering: C. 33:1460–1466.

Shiels, S.M., K.D. Solomon, M. Pilia, M.R. Appleford, and J.L. Ong. 2012. BMP‐2 tethered hydroxyapatite for bone tissue regeneration: Coating chemistry and osteoblast attachment. J. Biomedical Materials Res. Part A. 100:3117-3123.

Shimizu, H., H. Nakagami, N. Yasumasa, O.K. Mariana, M. Kyutoku, H. Koriyama, F. Nakagami, M. Shimamura, H. Rakugi, and R. Morishita. 2012. Links Between Hypertension and Osteoporosis: Benidipine Ameliorates Osteoporosis in Ovariectomized Hypertensive Rats Through Promotion of Osteoblast Proliferation and Inhibition of Osteoclast Differentiation. Current Cardiovascular Risk Reports. 6:274-280.

Valente, J., V. Gaspar, B. Antunes, P. Countinho, and I. Correia. 2012. Microencapsulated Chitosan-Dextran Sulfate Nanoparticles For Controled Delivery Of Bioactive Molecules And Cells In Bone Regeneration. Polymer. 54:5-15.

2011

Chen, F., X. Zhang, S. Sun, J.N. Zara, X. Zou, R. Chiu, C.T. Culiat, K. Ting, and C. Soo. 2011. NELL-1, an osteoinductive factor, is a direct transcriptional target of Osterix. PloS one. 6:e24638.

Ma, D., P. Tremblay, K. Mahngar, J. Collins, T. Hudlicky, and S. Pandey. 2011. Selective cytotoxicity against human osteosarcoma cells by a novel synthetic C-1 analogue of 7-deoxypancratistatin is potentiated by curcumin. PloS one. 6:e28780.

Medici, D., and B. Olsen. 2011. Conversion of vascular endothelial cells into multipotent stem-like cells. Patent Application US 20130078718 A1.

Zhang, X., C. Li, H. Gao, H. Nabeka, T. Shimokawa, H. Wakisaka, S. Matsuda, and N. Kobayashi. 2011. Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity. Cell Mol Biol Lett. 16:279-295.

2010

Zhang, X., A. Kovtun, C. Mendoza-Palomares, M. Oulad-Abdelghani, F. Fioretti, S. Rinckenbach, D. Mainard, M. Epple, and N. Benkirane-Jessel. 2010. SiRNA-loaded multi-shell nanoparticles incorporated into a multilayered film as a reservoir for gene silencing. Biomaterials. 31:6013-6018.

2008

Nathan, S.S., A.G. Huvos, J.E. Casas‐Ganem, R. Yang, I. Linkov, R. Sowers, G.R. DiResta, R. Gorlick, and J.H. Healey. 2008. Tumor interstitial fluid pressure may regulate angiogenic factors in osteosarcoma. J. Orthop. Res. 26:1520-1525.

Shimizu, H., H. Nakagami, M.K. Osako, R. Hanayama, Y. Kunugiza, T. Kizawa, T. Tomita, H. Yoshikawa, T. Ogihara, and R. Morishita. 2008. Angiotensin II accelerates osteoporosis by activating osteoclasts. The FASEB J. 22:2465-2475.

2004

Binnerts, M.E., X. Wen, K. Canté-Barrett, J. Bright, H.-T. Chen, V. Asundi, P. Sattari, T. Tang, B. Boyle, and W. Funk. 2004. Human Crossveinless-2 is a novel inhibitor of bone morphogenetic proteins. Biochemical and biophysical research communications. 315:272-280.

2002

Gutwein, L., and T. Webster. 2002. Osteoblast and Chrondrocyte Proliferation in the Presence of Alumina And Titania Nanoparticles. Journal of Nanoparticle Research. 4:231-238.

Kay, S., A. Thapa, K.M. Haberstroh, and T.J. Webster. 2002. Nanostructured polymer/nanophase ceramic composites enhance osteoblast and chondrocyte adhesion. Tissue engineering. 8:753-761.

Anti-BMPR2

Название	Код	Цена
Mouse BMPR2 Antibody	CP10368	по запросу

BMP-2 ELISA Kit, Human

Название	Код	Цена
Human BMP-2 ELISA Kit	CL0311	по запросу

Cryopreserved HOb All-in-One Kit

Название	Код	Цена
HOb Total Kit: Media, Subculture Reagents & Cells, Adult	406K-05a	по запросу
HOb Total Kit: Media, Subculture Reagents & Cells, Fetal	406K-05f	по запросу

Cryopreserved HOb, adult

Название	Код	Цена
Cryopreserved HOb	406-05a	по запросу

Cryopreserved HOb, fetal

Название	Код	Цена
Cryopreserved HOb	406-05f	по запросу

Osteoblast Basal Medium

Название	Код	Цена
Basal medium (contains no growth supplement).В Add GS before use.	416-500	по запросу

Osteoblast Differentiation Medium

Название	Код	Цена
Promotes cells to change from one type to another, more specialized	417D-250	по запросу

Osteoblast Growth Medium

Название	Код	Цена
All-in-one ready-to-use	417-500	по запросу
Growth medium without FBS	417F-500	по запросу

Osteoblast Growth Medium Kit

Название	Код	Цена
Basal medium & growth supplement sold together packaged separately	417K-500	по запросу

Osteoblast Growth Supplement

Название	Код	Цена
Added to Basal Medium to create Growth Medium	417-GS	по запросу

Proliferating HOb, adult

Название	Код	Цена
Proliferating HOb	407-25a	по запросу
Proliferating HOb	407-75a	по запросу
Proliferating HOb	407-6Wa	по запросу
Proliferating HOb	407-96Wa	по запросу

Proliferating HOb, fetal

Название	Код	Цена
Proliferating HOb	407-25f	по запросу
Proliferating HOb	407-75f	по запросу
Proliferating HOb	407-6Wf	по запросу
Proliferating HOb	407-96Wf	по запросу

Subculture Reagent Kit

Название	Код	Цена
100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	090K	по запросу

Extended Family Products

BMP-2

Название	Код	Цена
Human Bone Morphogenetic Protein-2	RP1053-10	по запросу
Human Bone Morphogenetic Protein-2	RP1053-1000	по запросу
Human Bone Morphogenetic Protein-2	RP1053-100	по запросу

Cyto-X Colorimetric Cell Counting Reagent

Название	Код	Цена
100 tests	028-S	по запросу
500 tests	028-01	по запросу

HOb RNA

Название	Код	Цена
Total RNA prepared from Human Osteoblasts, adult	406-R10a	по запросу
Total RNA prepared from Human Osteoblasts, adult	406-R25a	по запросу
Total RNA prepared from Human Osteoblasts, Differentiated, fetal	406D-R10f	по запросу
Total RNA prepared from Human Osteoblasts, Differentiated, fetal	406D-R25f	по запросу
Total RNA prepared from Human Osteoblasts, fetal	406-R25f	по запросу
Total RNA prepared from Human Osteoblasts, fetal	406-R10f	по запросу
Total RNA prepared from Human Osteoblasts, Stimulated, adult	406S-R10a	по запросу
Total RNA prepared from Human Osteoblasts, Stimulated, adult	406S-R25a	по запросу
Total RNA prepared from Human Osteoblasts, Stimulated, fetal	406S-R10f	по запросу
Total RNA prepared from Human Osteoblasts, Stimulated, fetal	406S-R25f	по запросу