Cat. No.: 118-50, 118-100, 118-1000
Source: Porcine insulin from pancreas
Physical Appearance: Porcine insulin powder
CAS No.: 12584-58-6
Quantity: 50 mg, 100 mg, 1000 mg
Purity: 95% by HPLC analysis
Potency: > 27 USPU/mg
Reconstitution:
1. Equilibrate bottle or vial of porcine insulin to room temperature.
2. Briefly spin down porcine insulin powder in the bottle or vial.
3. Dissolve porcine insulin by adding acidified water at pH 2.0 along the sides of bottle or vial to ensure full recovery of insulin:
5ml to 50mg vial
10ml to 100mg vial
100ml to 1000mg bottle
4. Mix porcine insulin gently to make a homogeneous solution.
5. Sterilize Porcine Insulin Stock Solution by filtering 10 ml through a syringe filter (0.2 μm pore size) and filtering 100ml through a 100ml filter unit (0.2 μm pore size).
6. Aliquot single use aliquots in sterile vials as stock solution.
Storage: Store stock vials of porcine insulin at -20°C in a non frost-free freeze. Do not thaw and refreeze insulin. Store thawed insulin at 4°C.
Suggested Usage: 1 to 10 microgram porcine insulin per milliliter of medium
Quality Control: