Anti-GAPDH: Mouse GAPDH Antibody |
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BACKGROUND Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4, 5).
REFERENCES
1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
3. Zheng, L. et al. (2003) Cell 114, 255-66.
4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
| Cat.No.: | CC10046 |
| Antigen: | Recombinant human GAPDH expressed in mammalian cells. |
| Isotype: | Mouse IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP 1:100 IHC 1:200 ICC 1:100 FACS n/d |
Predicted Molecular Weight of protein: | 35 kDa |
| Specificity/Sensitivity: | Detects endogenous GAPDH proteins without cross-reactivity with other family members. |
| Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
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