Anti-HA-Tag: Mouse HA-Tag Antibody |
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BACKGROUND Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The HA tag corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglunin(HA) which has been used extensively as a general epitope tag in expression vectors (1). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.
REFERENCES
1. Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
| Cat.No.: | CC10049 |
| Antigen: | A synthetic peptide containing the influenza hemagglutinin epitope |
| Isotype: | Mouse IgG |
Species & predicted species cross- reactivity ( ): | All |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC n/d ICC 1:100 FACS n/d |
Predicted Molecular Weight of protein: | n/d |
| Specificity/Sensitivity: | Detects HA-Tag proteins without cross-reactivity with other family members. |
| Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
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