Anti-HIP: Monoclonal Hsp70-Interacting Protein Antibody |
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BACKGROUND Hsp70-interacting protein, Hip is one of several cochaperones that regulate activities of the Hsp70 chaperone family. Hip functions to stabilize Hsp70 in an adenosine 5′-diphosphate (ADP)–bound conformation that appears to prolong its interaction with substrate. Hsp70 cycles between 2 conformations in its role as a chaperone, an adenosine triphosphate (ATP)–bound form with low affinity for substrate and an ADP-bound form with high affinity for substrate.1 Hip-Hsp70 interaction within multichaperone complexes involved in progesterone receptor assembly in mammals has been extensively examined. Hip associates transiently with Hsp70 and the progesterone receptor at an intermediate stage in assembly of the functional receptor complex, and Hip-Hsp70 association stabilizes the receptor in a conformation that prevents premature interaction with hormone in the cytosol. Before dissociating from the complex, Hip and Hsp70 also promote association of the cochaperone Hop along with Hsp90 and other proteins with the progesterone receptor to form the mature, hormone-receptive complex.2, 3
REFERENCES
1. Webb, M.A. et. Al: Cell Stress Chaperones 6: 247, 2001.
2. Smith, D. F. et al: Cell Dev. Biol. 11:45, 2000.
3. Kanelakis, K. C. et. Al: Biochemistry 39:14317, 2000.
2. Smith, D. F. et al: Cell Dev. Biol. 11:45, 2000.
3. Kanelakis, K. C. et. Al: Biochemistry 39:14317, 2000.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
Cat.No.: | CB19812 |
Antigen: | E. coli-expressed recombinant human Hip proteins. |
Isotype: | Mouse IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC (paraffin) n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 48 kDa |
Specificity/Sensitivity: | Detects endogenous human Hip proteins. Does not cross-react with other related members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
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