Anti-Lck: Mouse Lck Antibody |
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BACKGROUND Lck belongs to the Src-like non-receptor tyrosine kinase family with the typical Src family kinase structure: a unique amino terminal domain (Src homology 4 domain, SH4) followed by an SH3 domain, an SH2 domain, a kinase domain (SH1), and a carboxyl terminal negative regulatory domain (1). Lck activity is controlled by the interactions of SH2 and SH3 domains as well as tyrosine phosphorylation status of the activation loop (2, 3). Lck is recruited to the T cell receptor (TCR) complex upon stimulation and activates downstream tyrosine kinases to initiate T cell signaling (4). Lck is also found to be involved in the regulation of mitochondrial apoptosis pathways and may be responsible for some anticancer drug induced apoptosis (5, 6).
REFERENCES
1. Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990–8000.
2. Mustelin, T. and Taskén, K. (2003) Biochem J 371, 15–27.
3. Gervais, F.G. et al. (1993) Mol Cell Biol 13, 7112–21.
4. Straus, D.B. and Weiss, A. (1992) Cell 70, 585–93.
5. Belka, C. et al. (2003) Oncogene 22, 176–85.
6. Gruber, C. et al. (2004) Biochem Pharmacol 67,1859–72
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
| Cat.No.: | CC10051 |
| Antigen: | Recombinant human Lck expressed in Sf9 cells |
| Isotype: | Rabbit IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 56 kDa |
| Specificity/Sensitivity: | Detects endogenous Lck proteins without cross-reactivity with other family members. |
| Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
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