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IGFBP-3 ELISA Kit, Human


BACKGROUND Insulin-like growth factor binding protein-3 (IGFBP-3) is one of a family of six IGFBPs, multifunctional proteins that transport and stabilize IGFs in the circulation, modulate the effects of IGF on a variety of cellular functions, and may also regulate cells by IGF independent mechanisms. Initially purified from human plasma, IGFBP-3, a 40–45 kDa glycoprotein, is by far the most abundant IGFBP in the circulation, where it has a central role in regulating IGF bioavailability to the tissues by forming heterotrimeric complexes with IGF-I or IGF-II and an 85 kDa glycoprotein, the acid labile subunit. IGFBP-3 is also active in the cellular environment.1 It was showed that IGFBP-3 production is potently induced by TGF-beta. Moreover, it was also shown that the induction of IGFBP-3 in the mode of action of a variety of growth inhibitory proteins and agents, including p53, retinoic acid, vitamin D, anti-oestrogens, and TNF-alpha. The antiproliferative activity of IGFBP-3 may contribute to its anti-oncogenic effects.2

The effects of IGFBP-3 on IGF dependent cellular functions are complex, with both stimulatory and inhibitory actions reported, even within the one cell type. Several studies with cells lacking the type I IGF receptor have revealed that IGFBP-3 can be inhibitory to cell growth even in the absence of IGF signaling, raising the possibility of truly IGF independent effects of IGFBP-3. To date, no cell surface IGFBP-3 receptor that initiates an intracellular signaling cascade has been identified. It was shown that IGFBP-3 can stimulate phosphorylation of the cell surface receptor TGF-betaRI as well as phosphorylation of the TGF-beta signaling intermediates Smad2 and Smad3. Moreover, IGFBP-3 activates the promoter of the TGF-beta-responsive gene, PAI-1, which encodes plasminogen activator inhibitor-1 (PAI-1). PAI-1 blocks the activation of plasminogen to plasmin, a protease that causes partial fragmentation of IGFBP-3, thus limiting its ability to inhibit IGF action.3
 
REFERENCES  
1. Renehan, A.G. et al: Endocr Relat Cancer. 13:273-8, 2006
2. Burger, A.M. et al: Eur J Cancer 41:1515-27, 2005
3. Kuemmerle, J.F. et al: Am J Physiol Gastrointest Liver Physiol.;287:G795-802, 2004 
 
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Параметры

Cat.No.:
CL0386
Target Protein Species:
Human
Range:
156.2 pg/ml – 10000pg/ml
Specificity:
No detectable cross-reactivity
with any other cytokines
Storage:
Store at 4°C. Use within 6 months.
 
ELISA Kits are based on standard sandwich enzyme-linked immunosorbent assay technology. Freshly prepared standards, samples, and solutions are recommended for best results.


Документы



Публикации

2015
Miljus, G., V. Malenkovic, B. Dukanovic, N. Kolundzic, and O. Nedic. 2015. IGFBP-3/transferrin/transferrin receptor 1 complexes as principal mediators of IGFBP-3 delivery to colon cells in non-cancer and cancer tissues. Experimental and Molecular Pathology, 98:431-438.


НазваниеКодЦена
Human IGFBP-3 ELISA Kit CL0386 по запросу




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