GH: Human Growth Hormone |
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BACKGROUND Growth Hormone (GH) is an important growth factor made in and secreted throughout the body by the anterior pituitary gland. It stimulates growth and cell reproduction and regeneration in humans and other animals. The inability to appropriately produce or respond to GH results in diseases of decreased stature in children and adults. Although GH is used to treat several growth disorders, it is thought to be a very complex hormone with many of its functions yet to be uncovered.
Recombinant human GH is non-glycosylated protein, containing 192 amino acids, with a molecular weight of 22.2 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
Cat. No.:
RP1040
Alternative Name:
Somatotropin
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Recombinant human GH is lyophilized from 20 mM NaHCO3, pH 8.0.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity is determined by the ability to induce proliferation of Nb211 rat lymphoma cells for this effect and is typically 25-100 pg/mL.
AA Sequence:
MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFEEAYIPKE QKYSFLQNPQ TSLCFSESIP TPSNREETQQ KSNLELLRIS LLLIQSWLEP VQFLRSVFAN SLVYGASDSN VYDLLKDLEE GIQTLMGRLE DGSPRTGQIF KQTYSKFDTN SHNDDALLKN YGLLYCFRKD MDKVETFLRI VQCRSVEGSC GF
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
