BACKGROUND Resistin-Like Molecule-beta (RELM- β) is a member of a recently identified family of secreted proteins containing conserved cysteines in their C terminus. The RELM family consists of Resistin (also called FIZZ3), RELM-α (FIZZ1), and RELM-γ. Only Resistin and RELM-β were found in humans whereas all four RELM family members have been identified in rodents.
Recombinant human RELM-β is a non-glycosylated, disulfide-linked homodimer. Each monomer contains 89 amino acids, with the dimer having a total molecular weight of 19 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Cat. No.:
RP1160
Alternative Name:
FIZZ2
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Recombinant human RELM-β is sterile filtered and lyophilized from 0.1% TFA.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
There is no biological assay available at this time.
AA Sequence:
MQCSLDSVMD KKIKDVLNSL EYSPSPISKK LSCASVKSQG RPSSCPAGMA VTGCACGYGC GSWDVQLETT CHCQCSVVDW TTARCCHLT