SDF-1 α / CXCL12: Mouse Stromal Cell-Derived Factor-1 alpha |
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BACKGROUND Stromal Cell Derived Factor-1 alpha (SDF-1α), also called CXCL12, is one of two splice variants made by a wide variety of cells when stimulated by inflammatory cytokines such as, TNF, IL-1 or LPS. SDF-1α signals through the G protein-couple receptor, CXCR4, to recruit activated leukocytes. Human and mouse SDF-1α share 99% sequence identity.
Recombinant mouse SDF-1α is a non-glycosylated protein, containing 68 amino acids and having a molecular mass of 7.9 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
Cat. No.:
RP2046
Alternative Name:
CXCL12, PBSF
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Recombinant mouse SDF-1α is lyophilized from a concentrated (1mg/ml) sterile solution containing 10 mM acetic acid (AcOH).
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity as determined by its ability to chemoattract human monocytes at 50-100 ng/mL.
AA Sequence:
KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
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