TGF-β 1: Human Transforming Growth Factor beta 1 |
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BACKGROUND Transforming Growth Factor-beta 1 ( TGF-β1) is a member of the TGF-beta superfamily.
Параметры
Cat. No.:
RP1039
Alternative Name:
Differentiation inhibiting factor, cartilage-inducing factor
Source:
Human HEK 293 cells
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Human TGF-β1 is lyophilized from a sterile buffer containing 10 mM HCl + 50 ug BSA per mg/ml of protein.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 5 mM HCl + 50 ug/ml BSA at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity is measured by the dose-dependent inhibition of IL-4-induced proliferation of mouse HT-2 cells and is typically 0.04-0.2 ng/mL.
AA Sequence:
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
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