TPO: Human Thrombopoietin |
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BACKGROUND Thrombopoietin (TPO) is a growth factor that is produced by the liver and kidney. TPO acts through the TPO receptor to promote megakaryocyte maturation and differentiation, which leads to the production of platelets.
Recombinant human TPO is a non-glycosylated protein, containing the 174 amino acids of the receptor binding domain and having a molecular mass of 18.6 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
Cat. No.:
RP1005
Alternative Name:
Megakaryocyte Colony Stimulating Factor, c-MPL ligand, MGDF
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Recombinant human TPO is lyophilized from 10 mM sodium phosphophate buffer, pH 8.0.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity is determined by the dose-dependent stimulation of MO7e cells and is typically less than1 ng/ml.
AA Sequence:
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
