Anti-AMPK: Polyclonal AMPK-beta 1/2 Antibody |
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BACKGROUND AMPK is a serine/threonine protein kinase, which serves as an energy sensor in all eukaryotic cell types. Mammalian AMPK is a trimeric enzyme comprising a catalytic alpha subunit (63 kDa) and non-catalytic beta and gamma subunits. Multiple isoforms of each mammalian enzyme exist (alpha-1, alpha-2, beta-1, beta-2, gamma-1, gamma-2, gamma-3), each encoded by a different gene.1 AMPK activation strongly suppresses cell proliferation in non-malignant cells as well as in tumour cells. These actions of AMPK appear to be mediated through multiple mechanisms including regulation of the cell cycle and inhibition of protein synthesis, de novo fatty acid synthesis, specifically the generation of mevalonate as well as other products downstream of mevalonate in the cholesterol synthesis pathway.2 Cell cycle regulation by AMPK is mediated by up-regulation of the p53–p21 axis as well as regulation of TSC2–mTOR (mammalian target of rapamycin) pathway.3 The AMPK signalling network contains a number of tumour suppressor genes including LKB1, p53, TSC1 and TSC2, and overcomes growth factor signalling from a variety of stimuli (via growth factors and by abnormal regulation of cellular proto-oncogenes including PI3K, Akt and ERK). These observations suggest that AMPK activation is a logical therapeutic target for diseases rooted in cellular proliferation, including atherosclerosis and cancer.
REFERENCES
1. Kemp BE et al.: Structure, 15:1161-1163, 2007.
2. Zhang BB et al.: Cell Metabol. 9:407-416, 2009.
3. Thoreen C & Sabatini D: Cell Metabol. 1: 287-288, 2005.
2. Zhang BB et al.: Cell Metabol. 9:407-416, 2009.
3. Thoreen C & Sabatini D: Cell Metabol. 1: 287-288, 2005.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
Cat.No.: | CC1044 |
Antigen: | Sequence near the human AMPK-beta-1 carboxyl terminal. |
Isotype: | Affinity Purified Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions: | WB 1:1000 IP n/d IHC (Paraffin) n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 34 & 38 kDa |
Specificity/Sensitivity: | Detects endogenous levels of AMPK beta 1/2 proteins in various cell lysates. |
Storage: | Store at 4° C for frequent use; at -20° C for at least one year. |
*Optimal working dilutions must be determined by end user.
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