Anti-Blk: Mouse B Lymphoid Tyrosine Kinase Antibody |
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BACKGROUND The Src gene family has nine known members (Blk, c-Fgr, Fyn, Hck, Lck, Lyn, c-Src, c-Yes and Yrk), each encoding a cytoplasmic protein-tyrosine kinase (PTK) believed to be involved in signal transduction. These PTKs are expressed in a variety of cell types and participate in ligand-mediated signal transduction events. In B-lineage cells, multiple PTKs have been shown to be noncovalently coupled to membrane Ig-receptor complex, including p55blk, p59fyf, p53/56Lyn, and p56lck. More importantly, crosslinking of membrane immunoglobulin (mIg) by anti-Ig induces activation of these PTKs. One of the immediate consequences of PTK activation is tyrosine phosphorylation of several important intermediate effectors, such as phospholipase C-gamma, phosphatidylinositol 3-kinase, and GTPase activating protein, through which PTKs accomplish their role in signal transduction.1 Blk is exclusively expressed in B cells and thymocytes but not in mature T cells.2 Besides a role of Blk in B-cell receptor (BCR) signaling and B-cell development, the cellular functions of Blk are poorly defined. Expression of constitutively active Blk in the murine B-cell linage results in increased proliferation of progenitor B cells and eventually development of B-lymphoid lymphomas. The oncogenic capacity of Blk is kinase dependent because lymphoid tumors do not develop in mice expressing a catalytically inactive form of Blk.3
REFERENCES
1. Parsons, S.J. & Parsons, J.T.: Oncogene 23:7906-9, 2004
2. Saouaf, S.J. et al: J. Biol. Chem. 270:27072-8, 1995
3. Dymecki, S.M. et al: Sciences 247:332-6, 1990
2. Saouaf, S.J. et al: J. Biol. Chem. 270:27072-8, 1995
3. Dymecki, S.M. et al: Sciences 247:332-6, 1990
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
Cat.No.: | CP10024 |
Antigen: | Purified recombinant human Blk protein fragments expressed in E. coli. |
Isotype: | Mouse IgG1 |
Species & predicted species cross- reactivity ( ): | Human |
Applications & Suggested starting dilutions:* | WB 1:500 - 1:2000 IP n/d IHC 1:200 - 1:1000 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 55 kDa |
Specificity/Sensitivity: | Detects Blk proteins from B cell lysates without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
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