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Anti-Blk: Mouse B Lymphoid Tyrosine Kinase Antibody


BACKGROUND The Src gene family has nine known members (Blk, c-Fgr, Fyn, Hck, Lck, Lyn, c-Src, c-Yes and Yrk), each encoding a cytoplasmic protein-tyrosine kinase (PTK) believed to be involved in signal transduction. These PTKs are expressed in a variety of cell types and participate in ligand-mediated signal transduction events. In B-lineage cells, multiple PTKs have been shown to be noncovalently coupled to membrane Ig-receptor complex, including p55blk, p59fyf, p53/56Lyn, and p56lck. More importantly, crosslinking of membrane immunoglobulin (mIg) by anti-Ig induces activation of these PTKs. One of the immediate consequences of PTK activation is tyrosine phosphorylation of several important intermediate effectors, such as phospholipase C-gamma, phosphatidylinositol 3-kinase, and GTPase activating protein, through which PTKs accomplish their role in signal transduction.1  Blk is exclusively expressed in B cells and thymocytes but not in mature T cells. Besides a role of Blk in B-cell receptor (BCR) signaling and B-cell development, the cellular functions of Blk are poorly defined. Expression of constitutively active Blk in the murine B-cell linage results in increased proliferation of progenitor B cells and eventually development of B-lymphoid lymphomas. The oncogenic capacity of Blk is kinase dependent because lymphoid tumors do not develop in mice expressing a catalytically inactive form of Blk.3
 
REFERENCES  
1. Parsons, S.J. & Parsons, J.T.: Oncogene 23:7906-9, 2004
2. Saouaf, S.J. et al: J. Biol. Chem. 270:27072-8, 1995
3. Dymecki, S.M. et al: Sciences 247:332-6, 1990
  
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Параметры

Cat.No.:
CP10024
Antigen:
Purified recombinant human Blk protein fragments expressed in E. coli.
Isotype:
Mouse IgG1
Species & predicted
species cross-
reactivity ( ):
Human
Applications &
Suggested starting
dilutions:*
WB                  1:500 - 1:2000
IP                    n/d
IHC                  1:200 - 1:1000
ICC                  n/d
FACS               n/d
Predicted Molecular
Weight of protein:
55 kDa
Specificity/Sensitivity:
Detects Blk proteins from B cell lysates without cross-reactivity with other family members.
Storage:
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.

*Optimal working dilutions must be determined by end user.



Документы



НазваниеКодЦена
Mouse B Lymphoid Tyrosine Kinase Antibody CP10024 по запросу




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