1. Каталог >
  2. Клеточные линии, штаммы, среды >
  3. Клеточная биология >
  4. Антитела >

Anti-BLNK: Mouse BLNK Antibody


BACKGROUND BLNK is a cytoplasmic linker or adaptor protein that plays a critical role in B cell function and development. It functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways.1 The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. It also plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK, activation of NF-kB and NFAT, as well as AP1 activation. BLNK is an important regulator of BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes.2 However; it does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. It is critical in orchestrating the pro-B cell to pre-B cell transition and BCR-induced B-cell apoptosis. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia.3 
 
REFERENCES  
1. Fu, C. et al: Immunity 9:93-103, 1998
2. Wienands, J. et al: J. Exp. Med. 188:791-5, 1998
3. Nakayama, J. et al: Blood 113:1483-92, 2009
  
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Параметры

Cat.No.:
CP10415
Antigen:
Raised against recombinant human BLNK fragments expressed in E. coli.
Isotype:
Mouse IgG1
Species & predicted
species cross-
reactivity ( ):
Human, Mouse, Rat
Applications &
Suggested starting
dilutions:*
WB                  1:1000
IP                    1:25 - 1:50
IHC                  1:50 - 1:200
ICC                  1:50 - 1:200
FACS               1:50 - 1:200
Predicted Molecular
Weight of protein:
68 kDa
Specificity/Sensitivity:
Detects BLNK proteins in various cell lysate.
Storage:
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.

*Optimal working dilutions must be determined by end user.



Документы



НазваниеКодЦена
Mouse BLNK Antibody CP10415 по запросу




Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой