Anti-FGR: Mouse FGR Antibody |
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BACKGROUND Src family kinases (SFKs) consist of eight non-receptor tyrosine kinases (Src, Fyn, Yes, Lck, Lyn, Hck, FGR and Blk) that interact with the intracellular domains of growth factor/cytokine receptors, GPCRs and integrins. Members of the Src kinase family have a very similar domain structure with a high degree of homology in the SH1 (catalytic), linker, SH2 (p-Tyr binding), SH3 (protein-protein interaction) and SH4 (membrane association) domains. c-Src, Fyn and Yes are ubiquitously expressed, although high levels of c-Src are found in platelets, neural tissue and osteoclasts. For c-Src, auto-phosphorylation of Tyr416 and dephosphorylation of Tyr527 is required to switch the kinase from the inactive closed formation to the active open formation.1 c-Src can be inactivated by two kinases, c-Src kinase (CSK) and CSK homologous kinase (CHK), both of which phosphorylate Tyr527 of c-Src. The activity of the Src kinase family can also be regulated by phosphatases (e.g. SHP1), binding to adaptor proteins (e.g. Cbp) and proteasomal degradation. Src kinases are key upstream mediators of both the PI 3-K and MAPK signaling pathways, and have been shown to have important roles in cell proliferation, migration and survival.2
FGR is a Src-family member. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. FGR protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway.3
REFERENCES
1. Merkel, M. Et al: J. Biol. Chem. 277:7405-11, 2001
2. Hamilton, M.T. et al: Am J Physiol Endocrinol Metab 275: E1016-E1022, 1998
3. Kamei, Y. et al: Biochem. Int. 26:923-34, 1992
2. Hamilton, M.T. et al: Am J Physiol Endocrinol Metab 275: E1016-E1022, 1998
3. Kamei, Y. et al: Biochem. Int. 26:923-34, 1992
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
Cat.No.: | CP10104 |
Antigen: | Purified recombinant human FGR fragment expressed in E. coli. |
Isotype: | Mouse IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse, |
Applications & Suggested starting dilutions:* | WB 1:1000 IB 1:50 IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 55 kDa |
Specificity/Sensitivity: | Detects endogenous FGR proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
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