Anti-Foxa2: Mouse Foxa2 Antibody |
 |
BACKGROUND Foxa (previously termed HNF3) transcription factors comprise a subfamily of forkhead transcription factors, which includes Foxa1, Foxa2, and Foxa3 (previously termed HNF3α, HNF3β, and HNF3γ respectively). They have been found to play important roles in multiple stages of mammalian life, beginning with early development, continuing during organogenesis, and finally in metabolism and homeostasis in the adult.1
Foxa2 is required for the formation of the node and notochord, and in its absence severe defects in gastrulation, neural tube patterning, and gut morphogenesis result in embryonic lethality. Foxa1 and Foxa2 cooperate to establish competence in foregut endoderm and are required for normal development of endoderm-derived organs such as the liver, pancreas, lungs, and prostate. In post-natal life, members of the Foxa family control glucose metabolism through the regulation of multiple target genes in the liver, pancreas, and adipose tissue.2 Foxa2 is part of the transcriptional network downstream of the insulin receptor, and improves insulin resistance in peripheral tissues. Foxa2 is known to control the expression of many key genes involved in β-cell glucose sensing, such as the Pdx-1 gene. Moreover, Foxa2 plays a central role in insulin release via the regulation of genes coding for both Kir6.2 (inward rectifier potassium channel member 6.2) and Sur1 (sulfonylurea receptor 1) genes, the two subunits of the ATP-dependent K+ (KATP) channel. In brief, it appears that Foxa2 plays a pivotal role in key β-cell functions and physiology and glucose homeostasis.3
Foxa2 acts as a 'pioneer' factor opening the compacted chromatin enable chromatin access for other tissue-specific transcription factors. It acts through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. It binds DNA with the consensus sequence 5'[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]3' (By similarity).4
REFERENCES
1. Friedman, J.R. & Kaestner, K.H.: Cell Mol Life Sci. 63:2317-28, 2006
2. Ang, S.L.:Adv. Exp. Med. Biol. 651:58-65, 2009
3. Lantz, K. et al: J. Clin. Invest. 114:512-20, 2004
4. Wederell,E.D. et al: Nuclei. Acid Res.35:4549-64, 2008
2. Ang, S.L.:Adv. Exp. Med. Biol. 651:58-65, 2009
3. Lantz, K. et al: J. Clin. Invest. 114:512-20, 2004
4. Wederell,E.D. et al: Nuclei. Acid Res.35:4549-64, 2008
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
Cat.No.: | CP10108 |
Antigen: | Purified recombinant human Foxa2 fragments expressed in E. coli. |
Isotype: | Mouse IgG |
Species & predicted species cross- reactivity ( ): | Human |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC 1:200 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 48 kDa |
Specificity/Sensitivity: | Detects endogenous Foxa2 proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Документы
Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
